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cpg type tlr9 ligand  (InvivoGen)


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    InvivoGen cpg type tlr9 ligand
    Cpg Type Tlr9 Ligand, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 541 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cpg type tlr9 ligand/product/InvivoGen
    Average 96 stars, based on 541 article reviews
    cpg type tlr9 ligand - by Bioz Stars, 2026-06
    96/100 stars

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    Levels of Th1 and Th17 cell development after stimulation with SJL and B6.S DCs in the presence of various TLR ligands. Purified CD4+ T cells from VP2-TCR-Tg SJL mice were activated for 4 days with SJL or B6.S DCs in the presence of PBS, VP274–86 peptide, UV-TMEV, or live TMEV with or without TLR agonists: Pam3CSK4 (Pam) (TLR2 ligand; 10 ng/ml), LTA (TLR2 ligand; 1 μg/ml), poly(I:C) (TLR3 ligand; 50 μg/ml), LPS (TLR4 ligand; 100 ng/ml), CL087 (TLR7 ligand; 0.1 μg/ml), or CpG (TLR9 ligand; 0.1 μg/ml). The cells were then restimulated for 6 h with PMA and ionomycin prior to FACS analysis. The plots were gated on CD4+ T cells. The numbers in the FACS plots represent the percentages of IFN-γ+ and IL-17+ cells within the CD4+ cells. The presented data are representative of two separate experiments.

    Journal: Journal of Virology

    Article Title: The Level of Viral Infection of Antigen-Presenting Cells Correlates with the Level of Development of Theiler's Murine Encephalomyelitis Virus-Induced Demyelinating Disease

    doi: 10.1128/JVI.02471-14

    Figure Lengend Snippet: Levels of Th1 and Th17 cell development after stimulation with SJL and B6.S DCs in the presence of various TLR ligands. Purified CD4+ T cells from VP2-TCR-Tg SJL mice were activated for 4 days with SJL or B6.S DCs in the presence of PBS, VP274–86 peptide, UV-TMEV, or live TMEV with or without TLR agonists: Pam3CSK4 (Pam) (TLR2 ligand; 10 ng/ml), LTA (TLR2 ligand; 1 μg/ml), poly(I:C) (TLR3 ligand; 50 μg/ml), LPS (TLR4 ligand; 100 ng/ml), CL087 (TLR7 ligand; 0.1 μg/ml), or CpG (TLR9 ligand; 0.1 μg/ml). The cells were then restimulated for 6 h with PMA and ionomycin prior to FACS analysis. The plots were gated on CD4+ T cells. The numbers in the FACS plots represent the percentages of IFN-γ+ and IL-17+ cells within the CD4+ cells. The presented data are representative of two separate experiments.

    Article Snippet: The TLR7 ligand CL087 and the TLR9 ligand type B CpG oligonucleotide were obtained from InvivoGen (San Diego, CA) and used at 0.1 μg/ml and 10 μg/ml, respectively.

    Techniques: Purification